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Recent publications from the Ensign laboratory: Wampler,
D. A. and Ensign, S. A. 2005 " Photoheterotrophic
metabolism of Acrylamide by a Newly Isolated Strain of Rhodopseudomonas palustris" Appl.
Environ. Microbiol. 71, 5850-5857.
Boyd, J. M. and Ensign, S. A. 2005 “Evidence for
a Metal-Thiolate Intermediate in Alkyl Group Transfer from Epoxypropane
to Coenzyme M and Cooperative Metal Ion Binding in Epoxyalkane:CoM Transferase” Biochemistry,
44, 13151-13162.
Boyd,
J. M. and Ensign, S. A. 2005 “ATP-dependent Enolization
of Acetone by Acetone Carboxylase from Rhodobacter capsulatus” Biochemistry,
44, 8543-8553.
Boyd,
J. M., Ellsworth, H. and Ensign, S. A. 2004 “Bacterial acetone
carboxylase is a manganese-dependent metalloenzyme” The Journal
of Biological Chemistry, 279, 46644-46651.
Clark,
D. D., Boyd, J.M., and Ensign, S. A 2004 “The
Stereoselectivity and Catalytic Properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate
Dehydrogenase Are Controlled by Interactions between C-Terminal Arginine
Residues and the Sulfonate of Coenzyme M” Biochemistry, 43, 6763-6771.
Ensign,
S. A. and Allen, J. R. 2003 “Aliphatic Epoxide Carboxylation” Annual
Review of Biochemistry, 72, 55-76.
Nocek, B., Jang, S. B., Jeong, M. S., Clark, D. D., Ensign, S. A., Peters,
J. W†. 2002 “Structural basis for CO2 fixation by a novel
member of the disulfide oxidoreductase family of enzymes: 2-ketopropyl-Coenzyme
M Oxidoreductase/ Carboxylase” Biochemistry, 41, 12907-12913.
Sluis
Miriam. K., Larsen, Rachel A.., Krum, Jonathan. G., Anderson, Ruth.,
Metcalf, William. W. and Ensign, Scott A. 2002 "Biochemical,
molecular and genetic characterization of the ATP-dependent acetone carboxylases
of Rhodobacter capsulatus and Xanthobacter autotrophicus strain
Py2".
The Journal of Bacteriology, 184, 2969-2977.
Krum, Jonathan. G., Ellsworth, Heather, Sargeant, Ryan., Rich, Greg.,
and Ensign, Scott. A†. 2002 “Kinetic and Microcalorimetric
Analysis of Substrate and Cofactor Interactions in Epoxyalkane:CoM Transferase:
a Zn-Dependent Epoxidase”. Biochemistry, 41, 5005-5014.
Clark, Daniel. D. and Ensign, S. A. 2002 "Characterization of the
2-[(R)-2-hydroxypropylthio] ethanesulfonate Dehydrogenase from Xanthobacter strain Py2: Product Inhibition, pH-Dependence of Kinetic Parameters,
Site-Directed Mutagenesis, Rapid-Equilibrium Inhibition, and Chemical
Modification". Biochemistry, 41, 2727-2740.
Ensign, Scott. A. 2001 “Microbial Metabolism of Aliphatic Alkenes”,
Biochemistry, 40, 5845-5853.
Krum, Jonathan. G. and Ensign, Scott. A. 2001 "Evidence that a
linear megaplasmid in Xanthobacter strain Py2 encodes the enzymes of
aliphatic alkene and epoxide metabolism and Coenzyme M (2-mercaptoethanesulfonate)
biosynthesis" the Journal of Bacteriology, 183, 2172-2177
Krum, Jonathan G., and Ensign, Scott A. 2000 "Heterologous expression
of bacterial epoxyalkane:coenzyme M transferase and inducible coenzyme
M biosynthesis in Xanthobacter strain Py2 and Rhodococcus
rhodochrous B276." the Journal of Bacteriology, 182: 2629-2634..
Clark, Daniel D., Allen, Jeffrey R., and Ensign, Scott A. 2000 "Characterization
of Five Catalytic Activities Associated with the NADPH:2-Ketopropyl-coenzyme
M [2-(2-Ketopropylthio)ethanesulfonate] Oxidoreductase/Carboxylase of
the Xanthobacter Strain Py2 Epoxide Carboxylase System." Biochemistry,
39, 1294-1304.
Allen, Jeffrey R., Clark, Daniel D., Krum, Jonathan G. , and Ensign,
Scott A†. 1999 "A role for coenzyme M in a bacterial
pathway of aliphatic epoxide carboxylation" the Proceedings of the
National Academy of Sciences, 96:8432-8437..
Clark, Daniel D. and Ensign, Scott A. 1999 "Evidence for an inducible
nucleotide-dependent acetone carboxylase in Rhodococcus rhodochrous strain
B276" J. Bacteriol., 181, 2752-2758.
Allen, Jeffrey R. and Ensign, Scott A†. 1999 " Two
Short-Chain Dehydrogenases Confer Stereoselectivity for Enantiomers
of Epoxypropane
in the Multiprotein Epoxide Carboxylating Systems of Xanthobacter strain
Py2 and Nocardia corallina B276." Biochemistry, 38, 247-256.
Allen, Jeffrey R. and Ensign, Scott A†. 1998 "Identification
and characterization of epoxide carboxylase activity in cell extracts
of Nocardia corallina strain B276" the Journal of Bacteriology,
180, 2072-2078.
"
Ensign, Scott A.†, Small, Frederick J., Allen, Jeffrey R., and
Sluis, Miriam K. 1998 “New Roles for CO2 in the Microbial Metabolism
of Aliphatic Epoxides and Ketones" Archives of
Microbiology, 169, 179-187.
Allen, Jeffrey R. and Ensign, Scott A†. 1997 "Purification
to homogeneity and reconstitution of the individual components of the
epoxide carboxylase multiprotein enzyme complex from Xanthobacter strain
Py2." the Journal of Biological Chemistry 272, 32121-32128.
Holz, Richard C†., Small, Frederick J., and Ensign, Scott A†.
1997 "1H NMR Investigation of the [2Fe-2S]1- Containing "Rieske-Type" Protein
from Xanthobacter Strain Py2" Biochemistry, 36, 14690-14696.
Small, Frederick J. and Ensign, Scott A† 1997 "Alkene
Monooxygenase from Xanthobacter strain Py2: Purification and
Characterization of A Four-Component System Central to the Bacterial
Metabolism of Aliphatic
Alkenes" the Journal of Biological Chemistry 272, 24913-24920.
Sluis Miriam K. and Ensign, Scott A†. 1997 "Purification
and Characterization of Acetone Carboxylase from Xanthobacter strain
Py2" The Proceedings of the National Academy of Sciences, 94,
8456-8461.
Allen, Jeffrey R. and Ensign, Scott A†. 1997 "Characterization
of Three Protein Components Required for Functional Reconstitution of
the Epoxide Carboxylase Multienzyme Complex from Xanthobacter strain
Py2" J. Bacteriol., 179, 3110-3115.
Sluis Miriam K., Small, Frederick J., Allen, Jeffrey R. and Ensign,
Scott A†. 1996 "Involvement
of an ATP-Dependent Carboxylase in a CO2-Dependent Pathway
of Acetone Metabolism by Xanthobacter strain Py2" J. Bacteriol. 178,
4020-4026.
Allen, Jeffrey R. and Ensign, Scott A†. 1996 "Carboxylation
of Epoxides to ß-Keto Acids in Cell Extracts of Xanthobacter Strain
Py2" J. Bacteriol. 178, 1469-1472.
Ensign, Scott A†. 1996 "Aliphatic
and chlorinated alkenes and epoxides as inducers of alkene monooxygenase
and epoxidase activities
in Xanthobacter strain Py2" Appl. Environ. Microbiol. 62, 61-66.
Small, Frederick J. and Ensign, Scott A†. 1995 "Carbon
Dioxide Fixation in the Metabolism of Propylene and Propylene oxide
by Xanthobacter strain Py2" J. Bacteriol. 177, 6170-6175.
Seefeldt, Lance C†., Rasche, Madeline E., and Ensign, Scott A.
1995 "Carbonyl Sulfide and Carbon Dioxide as New Substrates and
Carbon Disulfide as a New Inhibitor of Nitrogenase." Biochemistry,
34, 5382-5389.
Ensign, Scott A†. 1995 "Reactivity of Carbon Monoxide Dehydrogenase
from Rhodospirillum rubrum with Carbon Dioxide, Carbonyl Sulfide, and
Carbon Disulfide." Biochemistry, 34, 5372-5381.
Small, Frederick J., Tilley, James K. and Ensign, Scott A†. 1995 "Characterization
of a New Pathway for Epichlorohydrin Degradation by Whole Cells of Xanthobacter strain
Py2." Appl. Environ. Microbiol. 61, 1507-1513.
Seefeldt, Lance C†. and Ensign, Scott A. 1994 "A Continuous
Spectrophotometric Activity Assay for Nitrogenase Using the reductant
Titanium (III) Citrate." Analytical Biochemistry, 221, 379-386.
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