Chemistry USU Professor, Alexander Boldyrev Receives Top State Award in Chemistry

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Lance Seefeldt is a part of an interuniversity team learning how plants are able to fix dinitrogen.  Their work is summarized in Acc Chem Res.

“I can’t cleave my protein at the TEV site!”
You make a simple recombinant protein harboring a TEV cleavage site so you can remove the fusion tag after purification and then you can’t seem to get the TEV to do its job…the Hevel lab comes to the rescue with a new protocol just published in Analytical Biochemistry.  Graduate student Brenda B.Suh-Lialam discovered that the way in which the cells are lysed impacts the ability of TEV to do its job. Recombinant proteins purified from cells lysed under harsh conditions could not be cleaved while mild lysis conditions yielded recombinant proteins that could easily be cleaved.  This method may be useful for cleaving other challenging target proteins that have the TEV protease
recognition site.

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